Influenza-Specific Antibody-Dependent Phagocytosis
Identifieur interne : 000430 ( Main/Exploration ); précédent : 000429; suivant : 000431Influenza-Specific Antibody-Dependent Phagocytosis
Auteurs : Fernanda Ana-Sosa-Batiz [Australie] ; Hillary Vanderven [Australie] ; Sinthujan Jegaskanda [Australie] ; Angus Johnston [Australie] ; Steven Rockman [Australie] ; Karen Laurie [Australie] ; Ian Barr [Australie] ; Patrick Reading [Australie] ; Marit Lichtfuss [Australie] ; Stephen J. Kent [Australie]Source :
- PLoS ONE [ 1932-6203 ] ; 2016.
Abstract
Immunity to human influenza A virus (IAV) infection is only partially understood. Broadly non-neutralizing antibodies may assist in reducing disease but have not been well characterized.
We measured internalization of opsonized, influenza protein-coated fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed influenza HA-specific ADP in healthy human donors, in preparations of intravenous immunoglobulin (IVIG), and following IAV infection of humans and macaques.
We found that both sera from healthy adults and IVIG preparations had broad ADP to multiple seasonal HA proteins and weak cross-reactive ADP to non-circulating HA proteins. The ADP in experimentally influenza-infected macaque plasma and naturally influenza-infected human sera mediated phagocytosis of both homologous and heterologous IAVs. Further, the IAV phagocytosed in an antibody-mediated manner had reduced infectivity
We conclude that IAV infections in humans and macaques leads to the development of influenza-specific ADP that can clear IAV infection
Url:
DOI: 10.1371/journal.pone.0154461
PubMed: 27124730
PubMed Central: 4849649
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Influenza-Specific Antibody-Dependent Phagocytosis</title><author><name sortKey="Ana Sosa Batiz, Fernanda" sort="Ana Sosa Batiz, Fernanda" uniqKey="Ana Sosa Batiz F" first="Fernanda" last="Ana-Sosa-Batiz">Fernanda Ana-Sosa-Batiz</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Vanderven, Hillary" sort="Vanderven, Hillary" uniqKey="Vanderven H" first="Hillary" last="Vanderven">Hillary Vanderven</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Jegaskanda, Sinthujan" sort="Jegaskanda, Sinthujan" uniqKey="Jegaskanda S" first="Sinthujan" last="Jegaskanda">Sinthujan Jegaskanda</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Johnston, Angus" sort="Johnston, Angus" uniqKey="Johnston A" first="Angus" last="Johnston">Angus Johnston</name><affiliation wicri:level="3"><nlm:aff id="aff002"><addr-line>Drug Delivery, Disposition and Dynamics laboratory, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Drug Delivery, Disposition 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Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>bioCSL Ltd, Parkville, Victoria, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>bioCSL Ltd, Parkville, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Laurie, Karen" sort="Laurie, Karen" uniqKey="Laurie K" first="Karen" last="Laurie">Karen Laurie</name><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Barr, Ian" sort="Barr, Ian" uniqKey="Barr I" first="Ian" last="Barr">Ian Barr</name><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Reading, Patrick" sort="Reading, Patrick" uniqKey="Reading P" first="Patrick" last="Reading">Patrick Reading</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Lichtfuss, Marit" sort="Lichtfuss, Marit" uniqKey="Lichtfuss M" first="Marit" last="Lichtfuss">Marit Lichtfuss</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J." last="Kent">Stephen J. Kent</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff006"><addr-line>Melbourne Sexual Health Centre, Central Clinical School, Monash University, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Melbourne Sexual Health Centre, Central Clinical School, Monash University, 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sort="Ana Sosa Batiz, Fernanda" uniqKey="Ana Sosa Batiz F" first="Fernanda" last="Ana-Sosa-Batiz">Fernanda Ana-Sosa-Batiz</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Vanderven, Hillary" sort="Vanderven, Hillary" uniqKey="Vanderven H" first="Hillary" last="Vanderven">Hillary Vanderven</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Jegaskanda, Sinthujan" sort="Jegaskanda, Sinthujan" uniqKey="Jegaskanda S" first="Sinthujan" last="Jegaskanda">Sinthujan Jegaskanda</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Johnston, Angus" sort="Johnston, Angus" uniqKey="Johnston A" first="Angus" last="Johnston">Angus Johnston</name><affiliation wicri:level="3"><nlm:aff id="aff002"><addr-line>Drug Delivery, Disposition and Dynamics laboratory, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Drug Delivery, Disposition and Dynamics laboratory, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash University, Parkville, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash University, Parkville</wicri:regionArea><wicri:noRegion>Parkville</wicri:noRegion></affiliation></author><author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>bioCSL Ltd, Parkville, Victoria, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>bioCSL Ltd, Parkville, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Laurie, Karen" sort="Laurie, Karen" uniqKey="Laurie K" first="Karen" last="Laurie">Karen Laurie</name><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Barr, Ian" sort="Barr, Ian" uniqKey="Barr I" first="Ian" last="Barr">Ian Barr</name><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Reading, Patrick" sort="Reading, Patrick" uniqKey="Reading P" first="Patrick" last="Reading">Patrick Reading</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff005"><addr-line>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Lichtfuss, Marit" sort="Lichtfuss, Marit" uniqKey="Lichtfuss M" first="Marit" last="Lichtfuss">Marit Lichtfuss</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J." last="Kent">Stephen J. Kent</name><affiliation wicri:level="4"><nlm:aff id="aff001"><addr-line>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff006"><addr-line>Melbourne Sexual Health Centre, Central Clinical School, Monash University, Melbourne, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Melbourne Sexual Health Centre, Central Clinical School, Monash University, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff007"><addr-line>ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Parkville</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="sec001"><title>Background</title><p>Immunity to human influenza A virus (IAV) infection is only partially understood. Broadly non-neutralizing antibodies may assist in reducing disease but have not been well characterized.</p></sec><sec id="sec002"><title>Methods</title><p>We measured internalization of opsonized, influenza protein-coated fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed influenza HA-specific ADP in healthy human donors, in preparations of intravenous immunoglobulin (IVIG), and following IAV infection of humans and macaques.</p></sec><sec id="sec003"><title>Results</title><p>We found that both sera from healthy adults and IVIG preparations had broad ADP to multiple seasonal HA proteins and weak cross-reactive ADP to non-circulating HA proteins. The ADP in experimentally influenza-infected macaque plasma and naturally influenza-infected human sera mediated phagocytosis of both homologous and heterologous IAVs. Further, the IAV phagocytosed in an antibody-mediated manner had reduced infectivity <italic>in vitro</italic>.</p></sec><sec id="sec004"><title>Conclusion</title><p>We conclude that IAV infections in humans and macaques leads to the development of influenza-specific ADP that can clear IAV infection <italic>in vitro</italic>. Repeated exposure of humans to multiple IAV infections likely leads to the development of ADP that is cross-reactive to strains not previously encountered. Further analyses of the protective capacity of broadly reactive influenza-specific ADP is warranted.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kilbourne, Ed" uniqKey="Kilbourne E">ED Kilbourne</name></author><author><name sortKey="Smith, C" uniqKey="Smith C">C Smith</name></author><author><name sortKey="Brett, I" uniqKey="Brett I">I Brett</name></author><author><name sortKey="Pokorny, Ba" uniqKey="Pokorny B">BA Pokorny</name></author><author><name sortKey="Johansson, B" uniqKey="Johansson B">B Johansson</name></author><author><name sortKey="Cox, N" uniqKey="Cox N">N Cox</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tricco, Ac" uniqKey="Tricco A">AC Tricco</name></author><author><name sortKey="Chit, A" uniqKey="Chit A">A Chit</name></author><author><name sortKey="Soobiah, C" uniqKey="Soobiah C">C Soobiah</name></author><author><name sortKey="Hallett, D" 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Wilson</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Australie</li></country><region><li>Victoria (État)</li></region><settlement><li>Melbourne</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Ana Sosa Batiz, Fernanda" sort="Ana Sosa Batiz, Fernanda" uniqKey="Ana Sosa Batiz F" first="Fernanda" last="Ana-Sosa-Batiz">Fernanda Ana-Sosa-Batiz</name></region><name sortKey="Barr, Ian" sort="Barr, Ian" uniqKey="Barr I" first="Ian" last="Barr">Ian Barr</name><name sortKey="Jegaskanda, Sinthujan" sort="Jegaskanda, Sinthujan" uniqKey="Jegaskanda S" first="Sinthujan" last="Jegaskanda">Sinthujan Jegaskanda</name><name sortKey="Johnston, Angus" sort="Johnston, Angus" uniqKey="Johnston A" first="Angus" last="Johnston">Angus Johnston</name><name sortKey="Johnston, Angus" sort="Johnston, Angus" uniqKey="Johnston A" first="Angus" last="Johnston">Angus Johnston</name><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J." last="Kent">Stephen J. Kent</name><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J." last="Kent">Stephen J. Kent</name><name sortKey="Kent, Stephen J" sort="Kent, Stephen J" uniqKey="Kent S" first="Stephen J." last="Kent">Stephen J. Kent</name><name sortKey="Laurie, Karen" sort="Laurie, Karen" uniqKey="Laurie K" first="Karen" last="Laurie">Karen Laurie</name><name sortKey="Lichtfuss, Marit" sort="Lichtfuss, Marit" uniqKey="Lichtfuss M" first="Marit" last="Lichtfuss">Marit Lichtfuss</name><name sortKey="Reading, Patrick" sort="Reading, Patrick" uniqKey="Reading P" first="Patrick" last="Reading">Patrick Reading</name><name sortKey="Reading, Patrick" sort="Reading, Patrick" uniqKey="Reading P" first="Patrick" last="Reading">Patrick Reading</name><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><name sortKey="Vanderven, Hillary" sort="Vanderven, Hillary" uniqKey="Vanderven H" first="Hillary" last="Vanderven">Hillary Vanderven</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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